Cytogenetic abnormalities detected by fluorescence in situ hybridization panel in chronic lymphocytic leukemia

Abstract

Objective To investigate the significance of fluorescence in situ hybridization (FISH) panel in detecting cytogenetic abnormalities in patients with chronic lymphocytic leukemia (CLL).Methods A panel of FISH probes [D13S25 (13q14.3),RB1 (13q14),ATM(1 1q22.3),CSP12(12p1 1.1-12q1 1.1) and p53 (17p13.1)] were performed in 21 cases with CLL.The cytogenetic features in correlation with clinical manifestation,other laboratory tests and prognosis were analyzed.Results Cytogenetic abnormalities were found in 13 of 21 patients with CLL (61.90 ％).The most frequent abnormality was del(13q14) (42.86 ％),followed by trisomy 12 (14.29 ％),del(11q22) (9.52 ％) and del(17p13) (9.52 ％).There was no significant relationship among cytogenetic abnormalities and sex,binet stages,expression of CD38,level of lactate dehydrogenase.Conclusion FISH with probe panel is a rapid,sensitive and accurate technique for detection of cytogenetic abnormalities in patients with CLL. Key words: Leukemia, lymphocytic, chronic; Fluorescence in situ hybridization; Chromosome