Cytogenetic abnormalities and the failure of development after round spermatid injections

Abstract

Objective To assess cytologic and cytogenetic abnormalities following round spermatid injection. Design Prospective analysis. Setting In vitro fertilization centers. Patient(s) Fourteen couples accepted to a round spermatid injection (ROSI) and preimplantation genetic diagnosis (PGD) program after appropriate counseling. Intervention(s) ROSI, PGD, with fluorescence in situ hybridization for chromosome enumeration. Main outcome measure(s) Cytologic and cytogenetic abnormalities in oocytes, zygotes, and blastomeres. Result(s) The fertilization rate following ROSI was 36%. Only 11 of 143 (7.7%) oocytes developed to have several blastomeres. Cytologic and cytogenetic abnormalities accounted for the vast majority of blockage at oocyte, zygote, and early mitotic division stages. Four biopsied embryos were normal. These and seven others were implanted, but no pregnancy was achieved. Conclusion(s) A PGD diagnosis for common aneuploidies and blastocyst stage transfer is feasible for ROSI cases. Failure with ROSI is cause primarily by chromosome abnormalities, so use of ROSI in assisted reproductive technologies should be limited.