Abstract
Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols, with or without a cerium chloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereas MG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.
Highlights
Microporous spheres have advantages in a range of applications, e.g., as vehicles for transplantation of cultured cells and materials for filling defects in human tissues [1,2]
The GPTMS/AcOH solution was added to the chitosan solution and the mixture was stirred at room temperature for 1 h
The porous structures were interconnected (Figure 3) and the pore sizes depended on the freezing temperature
Summary
Microporous spheres have advantages in a range of applications, e.g., as vehicles for transplantation of cultured cells and materials for filling defects in human tissues [1,2]. Shirosaki et al reported that chitosan-γ-glycidoxypropyltrimethoxysilane (GPTMS) porous bulk hybrids had excellent cytocompatibility with various cells [3,4,5]. It is initially difficult for the cells to migrate into porous bulk hybrids [6]. In accumulations of porous granules, the approximate sizes of the circular gaps between the granules, as well as the pore size, influence the cell behavior. The porous granules of diameter 2 mm give circulate gaps of diameter of approximately 310 μm. This space size is suitable for osteoconduction [7]
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