Abstract
Objective To develop a functionalized self-assembling peptide hydrogel.and investigate its biocompatibility with neural stem cells(NSCs).Methods The functionalized self-assembling peptide was made by mixing the self-assembling peptide RADA16 and peptide RADA16-IKVAV.The norphologieal features of the functionalized self-assembling peptide were studied by atom force microscope (AFM).NSCs were isolated from the cerebral cortex of neonatal rats and cultured in serum-free medium.NSCs were seeded on the surface of both RADA16 self-assembling peptide hydrogel(control group)and the funetionalized self-assembling peptide hydrogel(experimental group).The proliferation behavior of the cells was evaluated by CCK-8 assay as expressed by absorbance value.Cell migration was detected by laser scan confocal microscope(LsCM).Immunofluorescenee staining with Nestin,MAP2,GFAP,and CC-1 was used to assess the differentiation of NSCs.Results AFM showed that the functionalized self-assembling peptide hydrogel was made up of nanofibers with diameter of 20-30 nm and length of hundreds nanometers.In 6 days after cell seeding,the depth of cell migration in experimental group[(53.2±5.4)μm]was significantly higher than that in control group[(27.5 4-3.7)μm,P＜0.05].CCK-8 assay showed that experimental group displayed a significant increase of absorbance value compared with that of the control group after 1-week culture(P＜0.05).Immunofluorenscene analysis indicated that the differentiation ratio of neurons from NSCs in control group and experimental group were(22.44±3.52)% and(40.35±4.84)%respectively after 13-days cultare.The difference between the two groups was significant(P＜0.05).Conclusion The functionalized self-assembling peptide hydrogel has good cytocompatibility with NSCs. Key words: Hydrogel; Neural stem cell; Cytoeompatibility; Biomaterial
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