Cytocompatibility and in vivo biodegradation of graphene-modified chitosan 3D porous scaffold

Abstract

Interconnected porous chitosan scaffolds coated by chemically modified graphene (such as graphene oxide, GO) film have previously been prepared. In the present study, the attachment and proliferation of L929 on the surface of the composite scaffolds were reported and their in vivo biocompatible and biodegradability were evaluated. L929 cell culture on GO-coated chitosan scaffold surface with lower GO content promoted cell viability, but at higher content (1.0 mg/mL) a slight cytotoxicity appeared. The biodegradation rate of the composite scaffolds exhibited a content dependent behavior, decreased of remaining area from pure chitosan scaffold to the composite of 1.0 mg/mL GO content. The results demonstrated the high potential applications of GO-coating in fields of tissue engineering and drug delivery.