Abstract
The electrocatalytic properties of cytochrome P450 2C9 and the cytochrome P450 2C9/FAD and cytochrome P450 2C9/FMN complexes have been studied using a two-electrode system. The system consisted of an enzymatic catalyst electrode modified by the membrane-like compound didodecyldimethylammonium bromide (SPE/DDAB) and a measuring electrode, modified with carbon nanotubes (SPE/CNT). To study the effectiveness of electroenzymatic reactions catalyzed by cytochrome P450 2C9, the nonsteroidal anti-inflammatory drug diclofenac was used as a substrate. Cytochrome P450 2C9 catalyzes the stereospecific hydroxylation reaction to form 4′-hydroxydiclofenac. The metabolite 4′-hydroxydiclofenac was recorded at a potential E=+0.12 (relative to Ag/AgCl).The use of FAD and FMN as low-molecular mediators made it possible to increase the efficiency of electrocatalysis of the SPE/DDAB/CYP2C9/FAD system to 148±10% and SPE/DDAB/CYP2C9/FMN to 113±6% compared to SPE/DDAB/CYP2C9 (100±5%), and also increase the rate of the enzymatic reaction by 1.5 and 1.13 times, respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
