Cytochromes c1 of kinetoplastid protozoa lack mitochondrial targeting presequences

Abstract

We have used the polymerase chain reaction to amplify cDNA fragments that encode the amino-terminal sequences of cytochrome c 1 from two distantly related kinetoplastid species, Crithidia fasciculata and Bodo caudatus. Cloning and sequencing of these fragments have revealed that these proteins lack conventional mitochondrial targeting presequences.