Abstract
The addition of H2 to suspensions of chemolithotrophically grown Rhizobium japonicum strikingly increased the rates of reduction of cytochromes of the b and c-types. There was no effect of H2, however, on the cytochrome reduction rates of suspensions of heterotrophically cultured cells which do not show hydrogenase activity. An additional component whose difference spectrum appears to be similar to that of b-type cytochromes was found in chemolithotrophically grown R. japonicum. The behavior of this additional component in the presence and absence of H2 and KCN was investigated and it is concluded that it is involved in H2 metabolism.
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