Abstract
ESR measurements were performed for the reaction systems composed of t- or n-butylhydroperoxide and cytochrome P-450 (UT-2), purified from rat liver microsomes. On addition of n-butylhydroperoxide to the P-450 at pH 7.4, ESR signal due to a ferric low-spin species (g1=2.29, g2=2.24 and g3=1.96) was recorded. The observed g-parameters agreed well with those of a model thiolate-heme-iron(III)-peroxide complex, Fe(III)TPP(−S-TGE)(−OO-t-butyl) (g1=2.285, g2=2.198 and g3=l.959). In terms of the g-parameters, the new P-450 complex was concluded to be a F450-butyl peroxide adduct, in which a butyl peroxide anion ligates at the sixth position of heme iron(III).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
