Abstract
The identification of different anatomical regions of the periaqueductal gray matter of rats was addressed in the present study by using the histochemical staining for the mitochondrial enzyme cytochrome oxidase. At caudal and middle levels, cytochrome oxidase histochemistry clearly demonstrates the existence of four subdivisions: dorsal, dorsolateral, ventrolateral and medial, whereas in sections from the rostral periaqueductal gray matter only two concentric bands are identifiable on the basis of the degree of cytochrome oxidase activity.
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