Abstract
In a popular amperometric superoxide sensor, cytochrome c (Cyt c) is covalently immobilized onto a gold electrode previously covered with a self-assembled monolayer (SAM) of carboxyl-terminated alkanethiols. Therefore, a study was carried out to reveal the origins of the current response of such sensors. SAMs made of either 3,3′-dithiodipropionic acid or 11-mercaptoundecanoic acid and 11-mercaptoundecanol were used to immobilize either Cyt c or an inert protein onto gold electrodes. The resulting modified electrodes were comparatively investigated by cyclic voltammetry and calibrated for superoxide detection. The obtained results bring evidence that significant part (>70 %) of the current response of the Cyt c-modified electrodes to superoxide comes from direct oxidation of superoxide on gold (i.e. does not involve Cyt c).
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