Abstract
Background and methodology: In the current study magnetic poly(ethylene glycoldimethacrylate-N-methacryloyl-(L)-histidine methyl ester) poly(Egdma–Mah)) was used as ametal chelated affinity particles. Cu2+ ions loaded directly to MAH ligand of magnetic particlesfor the adsorption of cytochrome c (Cyt c) in a continuous flow system.
 Results: The maximumCyt c adsorption capacity on the magnetic particles and Cu2+-chelated magnetic particles were42 mg/g and 197 mg/g in phosphate buffer (pH 8.0), respectively. Cu2+ loading increased theCyt c adsorption capacity, significantly. Cyt c adsorption capacity decreased with increasedtemperature and with increasing magnetic field. According to reusability studies Cyt c moleculescould be reversibly adsorbed and desorbed five times. The binding isotherm was determined byscatchard analysis followed by application of Hill equation to the data obtained, then bindingconstant and nH Hill coefficient were calculated.
 Bangladesh Journal of Medical Science Vol.20(1) 2021 p.159-163
Highlights
The links between molecular biophysics and biochemistry and human diseases have been the subject of considerable scientific research effort for a number of decades
In this study m-poly(Egdma-Mah) was produced and metal-chelating ligand, Mah, and the active functional group, L-histidin is incorporated in the polymeric structure. This material has magnetic behaviour and it is used as an adsorbent to investigate Cyt c adsorption capacity in continuous flow system
Cyt c adsorption studies were investigated by other researchers[19,20,21]
Summary
The links between molecular biophysics and biochemistry and human diseases have been the subject of considerable scientific research effort for a number of decades. Among the affinity chromatography methods immobilized metal affinity chromatography (Imac) is a great one for seperation of biomacromolecules[1,2,3,4]. It is widely used for seperation of biomolecules based on analytical, laboratory and pilot-scale 5-7. Imac prove an useful technique for seperation of biomolecules by using affinities of materials which contain N, O, S as an electron donor atoms to chelated first row transition metal ions as a electron pair acceptor molecule . Cu2+ ions loaded directly to MAH ligand of magnetic particles for the adsorption of cytochrome c (Cyt c) in a continuous flow system.
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