Abstract
The present work was aimed to investigate the phylogenetic relationships of Pentatomidae based on mitochondrial Cyt b gene sequences. We sequenced and analysed a 440 bp fragment of Cyt b gene to assess the level of sequence divergence in different species of Pentatomid bugs collected from distant localities of Northern India. The data divulged an R-value of 1.19 suggesting transitions to be more than transversions. 18 sequences representing 10 species and 5 genera were finally analysed. The intraspecific divergence ranged from 0.2 to 7.2% with an average of 3.75 ± 3.0% whilst interspecific divergence ranged from 3.8 to 10.3% with an average of 7.49 ± 1.7% and intergeneric distance ranged from 12.1 to 20.2% with an average of 16.37 ± 2.2%. The percentage of divergence values revealed a clear pattern of increased nucleotide diversity from conspecific to congeneric to intra subfamily level using Cyt b gene fragment. Phylogenetic trees were constructed using Neighbor-Joining method that revealed the phylogenetic status of Indian Pentatomid bugs with other species from different geographical locations. The results were also confirmed with additional molecular parameter, nucleotide substitution and pairwise genetic distance.
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