Abstract
This chapter discusses the cytochemical techniques for studying the diffuse neuro-endocrine system, including electron immunocytochemistry, in situ hybridization, and binding-site analysis by in vitro autoradiography. Immunocytochemistry remains the method of choice for localizing the various structures within the diffuse neuro-endocrine system (DNES). At present, a variety of sophisticated immunocytochemical methods is available, and the technique most suited to the particular area of interest should be selected. Numerous cytochemical methods are being used for the study of the DNES. Unlike early methods that gave information only on the static system, the newer morphological techniques described in this chapter allows the concomitant analysis of the anatomy and dynamic status of this complex and ubiquitous system of cells and nerves. Application of these methods continues to yield new data on the functional roles of the components of the DNES, their distributions and interrelationships, and their contributions to disease processes.
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