Abstract
The colloidal iron (CI)-binding method can be used for the study of the cytochemical properties of nuclear chromatin in differentiated human lymphocytes. Growth stimulation of lymphocytes, either with phytohemagglutinin or by cell-crowding, resulted in a rapid increase of CI-binding per unit amount of DNA which is likely to reflect a change of the deoxyribonucleoprotein (DNP) complex of importance for gene activation. A similar increase of the CI-binding capacity of the DNP-complex was found to be induced in non-proliferating cells pretreated in solutions of increased alkalinity or increased ionic strength and after blocking the amino groups in histones with acetic acid anhydride. Increased CI-binding was therefore interpreted as reflecting an increased electronegativity of the DNP complex due to a decreased interaction between DNA and histones. Evidence was also obtained that conformational changes of the DNP complex played an important role in the CI-binding reaction.
Published Version
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