Abstract
(1) It has been demonstrated that a hyaluronic acid type of polysaccharide is present in the cytoplasm and nucleus of E. histolytica, when grown with various mono-associates. (2) The outer surface coat of the F-22 strain with streptobacillus associate contained collagen, which was absent in other cultures studied. (3) Variation of the acid polysaccharide content of the cytoplasm and in the nature of the outer surface coat appears to be more closely related to the type of associated organism rather than to strain differences. (4) Ceric ammonium nitrate was capable of bonding with the acid polysaccharide staining material of both cytoplasm and nucleus.
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