Abstract
BackgroundSalt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress.ResultsIn this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, A total of 48 redoxed peptides were identified for 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. (The proteomics data generated in this study have been submitted to the ProteomeXchange and can be accessed via username: reviewer_pxd027550@ebi.ac.uk, password: q9YNM1Pe and proteomeXchange# PXD027550.)ConclusionsThe results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.
Highlights
Salt stress is a major abiotic stress that limits plant growth, development and productivity
Changes of Ascorbic acid (AsA) and GSH in BvM14 leaves under salt stress Recent studies showed that AsA and GSH are major antioxidants in plant salt stress response (Navrot et al 2011; Lin et al 2020; Khan et al 2020), here we measured changes of two major antioxidants AsA and GSH at 0, 5, 10, 20, 30, 60 and 90 min after 0, 200, 400 mM NaCl treatments
The results clearly showed that salt stress caused significant cellular redox changes as early as 10 min after the treatment
Summary
Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. When plants are subjected to salt stress, it can induce osmotic stress, ionic stress, oxidative. Reactive oxygen species (ROS) are generated in the course of salt stress. Two ROS scavenging systems are mainly responsible for alleviation of salt stress-induced oxidative stress, i.e., enzymatic antioxidant system (e.g., glutathione S-transferase (GST), glutaredoxin (GR), superoxide dismutase (SOD) and catalase (CAT)) and non-enzymatic antioxidant system (e.g., ascorbate (AsA) and glutathione (GSH)) (Dave et al 2012; Farooq et al 2016; Jung et al 2019). Redox proteomic research in sugar beet response to salt stress is yet to be conducted
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