Cyclosporin partitions into phospholipid vesicles and disrupts membrane architecture

Abstract

Cyclosporin is a fungal metabolite demonstrating potent immunosuppressive activity both in vitro and in vivo, but the mechanism of action is poorly understood. Using [ 3H]dihydrocyclosporin C ([ 3H]CsC) we observed significant binding by mononuclear cells, erythrocytes and phosphatidyl choline (PC) vesicles which was reversible by the addition of excess CsA. Trypsin, pronase or heat treatments demonstrated that B cells and adherent cells express a protease-sensitive membrane binding site not observed on T cells. The nature of the interaction between CsA and the PC vesicles was studied using the membrane surface probe 1-anilino-8-naphthyl sulfonic acid (ANS −). ANS −-induced fluorescence was reduced by 24% in the presence of 4.75×10 −7 M CsA indicating that CsA displaces ANS − from the PC vesicles. CsA also effected a shift in the phase transition temperature of PC vesicles from 23°C to 19°C. Finally, the rate of concanavalin A (Con A)-induced cap formation by T lymphocytes was approximately doubled in the presence of 2.6×10 −5 M CsA. These data demonstrate that CsA partitions into phospholipid vesicle membranes and the plasmalemma of mononuclear cells resulting in an increased membrane fluidity.