Abstract
The influence of the fungoid undecapeptide cyclosporin A (CyA) on PGE 2 release from cultured rat aortic smooth muscle cells was investigated in this study. We found that CyA time and concentration dependently (ED 50: 500 ng/ml) inhibited PGE 2 release from the cells. CyA attenuated both basal and PGE 2 release evoked by angiotensin II (10 −10–10 −6M), arginine vasopressine (10 −10–10 −6M) and ionomycin (10 −9–10 −6M). CyA (1μg/ml) did not affect the conversion of exogenous arachidonic acid (1μM) into PGE 2. The inhibitory effect of CyA was neutralized by high concentrations of the calcium ionophore ionomycin (> 3×10 −6M). Taken together our results indicate that CyA inhibits both basal and vasoconstrictor evoked PGE 2 release from vascular smooth muscle by impairing the availability of free arachidonic acid rather than by inhibiting the conversion of arachidonic acid into PGE 2.
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