Cyclophosphane-induced in vitro phagocyte activity of peripheral blood leukocytes from healthy donors

Abstract

Event Abstract Back to Event Cyclophosphane-induced in vitro phagocyte activity of peripheral blood leukocytes from healthy donors Olga V. Lebedinskaya1*, Elena A. Lebedinskaya1, Anatoliy P. Godovalov1, Yana N. Troynich1 and Alexandra E. Malykina1 1 Acad. E.A. Wagner Perm State Medical Academy, Russia The aim of research was to study the effect of cyclophosphane (CP) on phagocyte activity of blood cells from healthy donors. Investigations were carried out with peripheral blood samples of 14 healthy donors. Blood samples were incubated for 1 h with CP (100; 10; 1; 0,10 and 0,01 μg/ml), then with formalinized sheep erythrocytes in 20 or 60 min at 37°C. All phagocyte parametre s of neutrophils and monocytes were counted. Low CP concentrations are found to increment the relative amount of phagocytizing neutrophils as compared with the control and data obtained at high doses. At CP concentrations of 100 μg/ml the amount of phagocytizing neutrophils comprised 40,00±3,69%, while at CP concentration of 0,01 μg/ml it was 59,88±3,72% (p<0,05). Introduction of 0,01 μg/ml CP also provoked the elevation of the phagocytizing monocyte number. CP applied in high doses resulted in lowering of phagocyte number as compared with the control. The absolute number of non-phagocytizing neutrophils under CP concentration of 100 μg/ml was statistically greater than under the dose of 0,01 μg/ml. Absolute amount of phagocytizing monocytes under the CP concentration of 100 and 10 μg/ml was statistically less than in control samples. While assessing the uptaking ability of phagocytes it was found that index of phagocytosis completeness was less than 1 with all of CP doses analyzed. Thus, cytostatic cyclophosphane modulates the leukocyte phagocyte activity. High CP doses reduce all known parameters of monocytes and neutrophils. This work was supported by RFBR grant №11-04-96037r_ural_a and administrative body of Perm Region. Keywords: cyclophosphane, phagocyte activity, healthy donors, Monocytes, Neutrophils Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Lebedinskaya OV, Lebedinskaya EA, Godovalov AP, Troynich YN and Malykina AE (2013). Cyclophosphane-induced in vitro phagocyte activity of peripheral blood leukocytes from healthy donors. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00840 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Olga V Lebedinskaya, Acad. E.A. Wagner Perm State Medical Academy, Perm, Russia, lebedinska@mail.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga V Lebedinskaya Elena A Lebedinskaya Anatoliy P Godovalov Yana N Troynich Alexandra E Malykina Google Olga V Lebedinskaya Elena A Lebedinskaya Anatoliy P Godovalov Yana N Troynich Alexandra E Malykina Google Scholar Olga V Lebedinskaya Elena A Lebedinskaya Anatoliy P Godovalov Yana N Troynich Alexandra E Malykina PubMed Olga V Lebedinskaya Elena A Lebedinskaya Anatoliy P Godovalov Yana N Troynich Alexandra E Malykina Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.