Abstract
Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19 A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents an original mammalian model to study FLAP-dependent leukotriene synthesis. In FLAP cDNA transfected HT29 cl.19 A cells, FLAP expression led to an increase in cyclooxygenase pathway products (mainly PGE 2) without an increase in 5-lipoxygenase metabolism. This increase in PGE 2 synthesis was associated with a cyclooxygenase-2 up-regulation in comparison to untransfected HT29 cl.19A cells. These results suggest a possible interaction between the two major pathways of arachidonic acid metabolism.
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