CYCLODEXTRIN MODIFIED MICELLAR LIQUID CHROMATOGRAPHY FOR DETERMINATION OF HYDROCHLOROTHIAZIDE AND CEFDITOREN PIVOXEL IN BIOLOGICAL FLUIDS AND TABLETS

Abstract

A hybrid mobile phase of β-cyclodextrin and sodium dodecyl sulfate was used as two pseudostationary phases for the separation and determination of hydrochlorothiazide (HCZ) and cefditoren pivoxil (CFP) in human plasma, human urine, and tablets. HCZ is indicated as a first line therapy for the treatment of hypertension and CFP is a cephalosporin which was commonly used to treat some throat and lung infections. The experimental conditions were optimized and validated based on ICH Q2R1 guidelines in aqueous and biological samples to detect both analytes at 260 nm within 8.0 min by isocratic mobile phase of SDS (25.0 mmol L−1) and β-CD (0.5 mmol L−1) dissolved in a phosphate buffer (67.0 mmol L−1, pH 9.4), and was mixed with 15% (v/v) methanol in the presence of 1.5 mL min−1 flow rate and 30.0°C column temperature. Resolution of more than 2.5 between analytes was successfully achieved. The linearity of HCZ and CFP was achieved in the ranges of 1.55–40.70 and 0.74–20.14 ng mL1, respectively. Limits of detection (LOD) 0.63 and 0.31 ng mL−1 and limits of quantification (LOQ) 2.05 and 1.10 ng mL−1 for HCZ and CFP were obtained, respectively. In order to avoid long extraction and preconcentration and deproteinization steps, a direct injection of real biological samples was successfully applied giving adequate recoveries ranged between 98.12%–102.12% and relative standard deviations (RSD) were less than 2.54%. The selectivity and sensitivity achieved was attributed to the possibility of four point competition interactions among analytes, pseudostationary phases, and modified C18. The advantages of cyclodextrin modified micellar liquid chromatography (CDMLC) for determination of drugs could strongly participate for high-throughput scanning of HCZ or CFP tablets and their combined chemotherapies in biological samples.