Abstract
ObjectiveThis study aims to investigate the expression and significance of p57kip2 and cyclinD1 in gastric cardia adenocarcinoma (GCA). p57kip2 is a negative regulator in the cell cycle. On the contrary, cyclinD1 is a positive regulator of cell cycle progression.MethodsThirty-two cases of GCA tissues and adjacent non-cancerous tissues were collected for this study. Immunohistochemistry and fluorescence qualitative PCR was used to determine the level of p57kip2 and cyclinD1 in GCA and its adjacent non-cancerous tissues. Furthermore, the correlation between the mRNA/protein and GCA clinical pathologic parameters were analyzed, and the relationship of p57kip2 and cyclinD1 in GCA were also evaluated.ResultsThe expression of p57kip2 significantly lower in GCA (P = 0.036), and there was a significant correlation in the different degrees of differentiation (P < 0.05). Furthermore, median survival time was 41 months for patients with high mRNA expression of p57kip2. This was longer compared to patients with low mRNA expression of P57kip2 (37 months, X2 = 4.788, P = 0.029).The expression of cyclinD1 was significantly higher in GCA(P = 0.002), and was significant correlated to clinical stage(P<0.05). Median survival time was 34 months in patients with high mRNA expression of cyclinD1, which was shorter than in patients with low expression of cyclinD1 mRNA (41 months, X2 = 4.071, P = 0.044). The protein expression of p57kip2 was not correlated to the protein expression of cyclinD1 (P = 0.55).ConclusionThe expression of p57kip2 and cyclinD1 are likely to suppress or promote the tumorigenesis and progression of GCA.
Highlights
In many parts of northern China, gastric cardia adenocarcinoma (GCA) is a common cancer disease [1]
This study aims to investigate the expression and significance of p57kip2 and cyclinD1 in gastric cardia adenocarcinoma (GCA). p57kip2 is a negative regulator in the cell cycle
At protein level, 6 and patients were positive for p57kip2 in GCA and its adjacent non-cancerous tissues, respectively; while and 5 patients were positive for cyclinD1 in GCA and its adjacent non-cancerous tissues, respectively
Summary
In many parts of northern China, gastric cardia adenocarcinoma (GCA) is a common cancer disease [1]. The regional distribution of GCA is different from gastric and esophageal cancer [3]. These should be studied as an independent disease. Tumors can be caused by cells that have unlimited autonomous division and proliferation. Disorder in cell cycle progression can lead to a chaotic process. The regulation of different checkpoints in cell cycle progression is recognized as the cell cycle regulation mode. An www.impactjournals.com/oncotarget uncontrollable checkpoint would lead to the manifestation of a tumor, and almost all functions of oncogenes and cancer suppressor genes are correlated to cell cycle. As a positive regulator of cell cycle progression, cyclinD1 overexpression may accelerate G1/S progression, accelerating cell proliferation [5]
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