Abstract
Steroidogenic acute regulatory protein (StAR protein) has been believed playing important role in steroid hormone production. Our previous results indicate that protein expression and kinase activity of cyclin-dependent kinase 5 (Cdk5) are under luteinizing hormone receptor/cAMP control and regulates mouse androgen production through StAR protein phosphorylation and stabilization. The aim of this study is to understand that whether the Cdk5 regulation on androgen production can be happened in ovary thecal cells and responds to their androgen production. This is the pioneer report indicating the physiological function of Cdk5 in ovary cells. The results showed that Cdk5 and its regulator, p35, were abundant in primary mouse thecal cells. Furthermore, we found that the protein expressions of Cdk5 and p35 and kinase activity of Cdk5 in thecal cells were all controlled by human chorionic gonadotropin (hCG). Taking advantage of cAMP-related blockers, we identified that cAMP-related pathway was involved in hCG-dependent regulation on Cdk5/p35 in thecal cells. Specific Cdk5 inhibitor could significantly decrease the phosphorylation and protein levels of StAR in thecal cells. Finally, Cdk5 inhibitor could also diminish androstenedione production in thecal cell culture medium. In conclusion, these results first elucidate the physiological function of Cdk5 in steroid production of mouse ovary. (poster)
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