Cyclin D1 polymorphism and cervical cancer risk

Abstract

5050 Background: It is proposed that the cyclin D1 is to serve as an active switch in the regulation of continued cell cycle progression. Though many investigators have reported a relationship between the A/G870 polymorphism in exon 4 of the cyclin D1 gene and the susceptibility to carcinomas of various origins, there are few reports of that in cervical cancer. Our aim was to investigate the association of cyclin D1 single nucleotide polymorphism with susceptibility to cervical cancer in Korean women. Methods: The study was carried on 233 cervical cancer patients (cases) and 334 patients with benign disease (controls) who received surgical treatment at Seoul National University Hospital. By polymerase chain reaction and restriction fragment length polymorphism method, cyclin D1 genotyping was carried out. Results: Of the 233 cervical cancer patients, 186 (79.8%) had squamous cell carcinoma and 47 (20.2%) had other cell types of carcimoma. The analysis of the cases showed that the allelic and genotypic frequencies were similar in the patients with squamous cell carcinoma and those with other types of carcinoma. However the allelic frequencies of the cases (A, 0.54; G, 0.46) were significantly different from those of the controls (A, 0.49; G, 0.51) (p = 0.025). The G/G genotype was significantly less frequent among cases (6.9%) than among controls (16.2%) (p = 0.001). The regression analysis after adjusting for age showed that the A/G and A/A genotype had 3.02 (95% confidence interval [CI] = 1.54–5.93) and 2.44 (CI = 1.33–4.47) times increased risk for the development of cervical cancer compared to the G/G genotype. When analyzed with combining the A/G and A/A genotype, A/G + A/A genotype had 2.57 (CI = 1.42–4.67) times increased risk for the cervical cancer compared to the G/G genotype. Conclusions: Determination of the cyclin D1 single nucleotide polymorphism may be applicable to identify individuals with increased risk for cervical cancer in Korean women. No significant financial relationships to disclose.