Abstract
Succinic dehydrogenase activity was assayed in isolated rat liver mitochondria. Rats had been exposed for at least two weeks to a 24 hour feeding-fasting schedule, 5 hours feeding, 19 hours fasting. Enzyme activity was determined at nine specific hours over a 24 hour period. Lowest enzyme activity occured six hours after the feeding cages had been closed. The highest activity, which was 158 percent greater than the lowest activity, occurred during the next feeding period. It is concluded that time of sacrifice is an important consideration when determining succinic dehydrogenase activity.
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