Abstract
The present study further analyse the presence of cyclic nucleotide-gated (CNG) channels and its involvement in the nitrergic urethral relaxation. Our functional results confirm and extend to the rat those previously found in the sheep urethra [1]. Moreover, rat urethra is even more sensitive to the effect of L-cis-diltiazem, a selective inhibitor of CNG channels. Double-labelling immunofluorescence was performed on fixed sections (30 μm) of rat urethra to visualize CNG1 subtype channels (goat anti-rat CNG1//FITC-conjugated donkey anti-goat) and its colocalization with either neuronal NOS(rabbit anti-rat nNOS//Rhodamine-conjugated donkey anti-rabbit) or vimentin(mouse anti-vimentin//Rhodamine-conjugated donkey anti-mouse) positive cells. Micrographs of the from 2nd International Conference of cGMP Generators, Effectors and Therapeutic Implications Potsdam, Germany, 10–12 June, 2005
Highlights
Cyclic GMP-gated channels are co-localized with a population of Interstitial cells, but not with neuronal NOS, in the rat urethra
The present study further analyse the presence of cyclic nucleotide-gated (CNG) channels and its involvement in the nitrergic urethral relaxation
Double-labelling immunofluorescence was performed on fixed sections (30 μm) of rat urethra to visualize CNG1 subtype channels and its colocalization with either neuronal NOS- or vimentin- positive cells
Summary
Cyclic GMP-gated channels are co-localized with a population of Interstitial cells, but not with neuronal NOS, in the rat urethra. Interstitial cells, but not with neuronal NOS, in the rat urethra Email: Domingo Triguero* - dtriguer@vet.ucm.es * Corresponding author from 2nd International Conference of cGMP Generators, Effectors and Therapeutic Implications Potsdam, Germany, 10–12 June, 2005
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