Abstract
AbstractThe application of a combined solid phase extraction (SPE)/clean‐up procedure to the isolation of a purified fraction containing all the monomeric cyclic fatty acid methyl esters is described. Extraction of the nonpolar lipid components from nonurea‐adducting (NUA) filtrates is performed on a reverse phase octadecyl bonded silica minicolumn. Stepwise elution of the SPE‐retained materials through silica gel using several solvents allowed the separation of a pure fraction containing the cyclic monomers that can be used for a more reliable quantitative estimation of these compounds in edible fats and oils.
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