Cyclic di-GMP signalling in the virulence and environmental adaptation of Xanthomonas campestris

Abstract

This paper appeared in Ryan et al. (2007) Mol Microbiol 63(2): 429–442. doi:10.1111/j.1365-2958.2006.05531.x The authors have been made aware of issues with Fig. 1 and Supplementary Fig. S4 of this paper. In Fig. 1, the same leaf image labelled ‘ddH2O control’ was used both in this paper and in McCarthy et al. (2008) Mol Plant Pathol 9(6): 819–824 to represent the negative (un-inoculated) control. We believe this duplication occurred as the data sets for these two papers were generated from assays carried out at the same time. We have replaced this panel of Fig. 1 to address this issue of image duplication between the two articles. In Supplementary Fig. S4, errors in construction have led to duplication of images between columns 1 and 3, although the findings as described within the text of the paper are correct. In order to resolve this issue, we have repeated this experiment (with essentially the same outcome) and constructed a replacement Figure. The original quantitative data taken at the time of these experiments was provided to the journal for review. None of the conclusions of the paper are altered. This correction has been submitted by J. L. Tang and both corresponding authors (J. L. T. and J. M. D.) take full responsibility for the original errors and authenticity of the correction. We sincerely apologise to the readers for the errors and appreciate the opportunity to correct the scientific record. The effects of mutation of genes encoding cyclic di-GMP signalling proteins on the virulence of Xcc to Chinese Radish. The virulence of each mutant was tested by measurement of the lesion length after bacteria were introduced into the vascular system of Chinese radish by leaf clipping. A. Representative virulence assays for (from left to right) Xcc wild type, rpfG (XC2335::Tn5gusA5) mutant, rpfG deletion mutant and negative control (H2O). B. Mutation of 13 genes (XC0249, XC0420, XC0637, XC1036, XC1411, XC1476, XC1582, XC1775, XC2324 (pdeA), XC1841, XC0831, XC2335 (rpfG) and XC3163 gave a significant reduction in virulence in repeated tests. Supplementary Fig. 4. Effects of mutation of genes encoding cyclic di-GMP signalling proteins on biofilm formation by Xcc. Bacteria were grown in L medium in borosilicate glass tubes and incubated without shaking at 30°C for 24 h before crystal violet staining (please note XC1824, XC1841 and XC3800 are not shown but have no difference phenotype to wild type).