Abstract
A competitive protein-binding method has been used for cyclic AMP (cAMP) assay in teleost fishes. In plasma as in intestinal mucosa the basal values were unaffected by hypophysectomy (in goldfish) and by changes in salinity (in trout and flounder). In vivo, adrenaline and theophylline injections increased cAMP levels in plasma but had no effect on intestinal mucosa. Also in vitro, cAMP content of this tissue was not changed by adrenaline nor by isoproterenol and it was concluded that this is due to the absence of β-receptors. Incubation (15 or 30 min) with theophylline produced a large increase in cAMP concentration in intestinal mucosa. A consistent elevation was obtained with 1 m M theophylline and the maximum response was observed with 10 m M. These preliminary results are discussed in relation to the physiological effects of cAMP on water and ion metabolism in fishes.
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