Cyclic AMP formation of isolated human corpora lutea in response to HCG — Interference by PGF 2α

Abstract

Human corpora lutea of defined ages were excise at operation cut into pieces and incubated in the presence of HCG, PGF 2α and PGE 2 alone or in combination. Following incubation cAMP formation in tissue and medium was determined. HCG-stimulated tissue cAMP content was most pronounced at a corpus luteum age of 7–10 days after ovulation. This stimulation was antagonized by PGF 2α in corpora lutea older than 6 days. PGE 2 stimulated cAMP formation per se and this effect was more pronounced when HCG and PGE 2 were combined. A possible role for PGF 2α as a luteolytic substance in the human is suggested.