Abstract
PGE 2 produced a marked and dose-related increase in cAMP content of cultured bone cells and in the release of cAMP into the incubation medium. The amount of cAMP released from the cells by PGE 2 was proportional to the cellular concentration, and was dependent upon the time of incubation with PGE 2. The cAMP levels released into the media increased slowly at a linear rate during a 60 min treatment with PGE 2. This release was blocked by theophylline, probenecid, ouabain and dinitrophenol, suggesting that the release of cAMP was not a simple diffusive process and required energy. SC-19220 reduced the formation of cAMP more than the release, suggesting that the formation and the release may arise from separate events. Inability of D600 to inhibit PGE 2-induced release of cAMP indicates that the release does not require calcium.
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