Abstract
Abstract The induction of acrosome reaction (AR) happens when starfish spermatozoa encounter the egg jelly (EJ). This complex process involves different signal transduction pathways, such as elevation of cAMP and the activation of protein kinase A (PKA). The specific inhibitors of PKA (H89 and KT5720) have been shown to inhibit the EJ-induced Ca2+ elevation and AR. By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ. The specific PKA inhibitors effectively inhibit phosphorylation of these proteins, suggesting an involvement of PKA on EJ-induced AR.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
