Abstract
Polycations such as histone and protamine sulfate increase the efficiency of retention of cAMP-binding protein complexes on cellulose ester membrane filters. A 40,000 dalton cAMP-binding protein from calf ovaries was not retained on the membrane filters when assayed above pH 5.5. The addition of histone or protamine sulfate to the cAMP-binding assay completely abolished the pH dependency and resulted in retention of the complex. The retention of larger cAMP-binding proteins as well as the ovarian cAMP-dependent protein kinases showed little effect of histone at low pH values while a significant enhancement was observed above pH 7.0.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
