Abstract
Study evaluated the role of cAMP modulator (Forskolin and 3-isobutyl-1- methyl xanthine) supplementation on developmental competence of bovine oocytes. Cumulus oocyte complexes recovered from bovine ovaries of unknown reproductive status were used for the study. Oocytes retrieved by aspiration method were graded based on cumulus cell distribution and culture grade oocytes were selected for the study. A total of 414 culture grade oocytes were taken and divided into two groups. Group I constituted of 201 oocytes in which pre-maturation was carried out for a period of 2 h. In group II, 213 oocytes were selected in which normal maturation was carried out. Maturation was assessed after 24h of culture in CO2 incubator maintained at 38.5o C in 95 per cent humidified atmosphere of 5 per cent CO2 . Fertilisation was carried out using frozen thawed semen and the presumptive zygotes were then transferred to culture media and cleavage was assessed 48 h after insemination. A significantly higher maturation rate (p ?0.05) was observed in group I compared to group II (86.85 ± 1.19 vs 79.88 ± 2.67). There was a highly significant increase (p ?0.01) in cleavage rate in group I (65.92 ± 1.23) compared to group II (59.29 ± 1.50). A higher fertilisation rate was observed in group I (75.35 ± 1.19) than group II (71.88 ± 2.56). It could be concluded that pre-maturation with cAMP modulators improved the developmental competence of bovine oocytes.
Highlights
Study evaluated the role of cAMP modulator (Forskolin and 3-isobutyl-1- methyl xanthine) supplementation on developmental competence of bovine oocytes
The cAMP produced from cumulus cells (CC) is transferred to oocytes via gap junctions meaning gap junctional communication plays a major role in transport
Oocyte recovery rate found in the study was is in accordance with Rakshitha (2019), who obtained recovery rate as 86.44 per cent
Summary
Study evaluated the role of cAMP modulator (Forskolin and 3-isobutyl-1- methyl xanthine) supplementation on developmental competence of bovine oocytes. It could be concluded that pre-maturation with cAMP modulators improved the developmental competence of bovine oocytes. It is a major strategic research tool in developmental and reproductive biology (Albuz et al, 2010) It involves gathering of immature oocytes from antral follicles and its subsequent culture under laboratory conditions to generate metaphase II oocytes. Oocytes retrieved for in vitro studies have not got enough time for synthesis and reorganization because they are taken out of antral follicles at different developmental stages. They won’t be competent enough to hold future embryo. Up on removal of oocytes from follicles, the gap junctional communication will be altered leading to drastic reduction in level of cAMP which will eventually culminate in activation of maturation promoting factor thereby resumption of meiosis (Pan and Li, 2019)
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