Abstract
Cloned segments of Rhizobium genomic DNA encoding sequences for cyclic AMP synthesis (Adenyl cyclase) were identified from a gene bank of R. meliloti. The cloned DNA weakly complemented Escherichia coli Δcya mutants, promoting the expression of adenyl cyclase activity and the accumulation of cAMP. The complemented Δcya clones were also capable of inducing a number of cAMP dependent carbon utilization operons but displayed an altered pattern of catabolite repression control. A common 5.4 Kb Bg/II DNA fragment, with internal EcoR1, BamH1 and PstI sites and inserted in different orientations was identified in the complementing recombinant plasmids. The identification of this DNA sequence will be a useful tool in elucidating the role of cAMP in these agronomically important bacteria.
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