Abstract
Electrofusion was conducted to use the embryogenic callus cells of satsuma mandarin (Citrus unshiu cv. ‘Juman’ unshiu) and mesophyll cells of yuzu (C. junos) and lemon (C.limon cv. ‘Eureka’ lemon). Two plants were regenerated from the combination of satsuma mandarin and yuzu, and one plant from that of satsuma mandarin and lemon. The plants had 18 chromosomes (2n=2×=18 in each parent), and showed the same nuclear rDNA fragment pattern as that of the mesophyll parent. The mitochondrial DNA analysis showed the presence of specific yuzu or lemon bands in addition to specific satsuma mandarin bands. From these results with morphological evidence, the regenerated plants were considered as cybrids with recombined mitochondrial DNA.
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