Abstract
The Thamnotoxkit F™ was evaluated for detecting cyanobacterial toxins compared with the rat hepatocyte test and mouse test. This test kit is a 24-h bioassay using larvae of the freshwater anostracan crustacean Thamnocephalus platyurus hatched from cysts. Thirteen freeze-dried cyanobacterial samples from freshwaters of Hungary, Germany, and Brazil, and strains from the culture collection of the University of Helsinki were tested and analyzed for microcystins and anatoxin a by high performance liquid chromatography and in some cases, the enzyme-linked immunosorbent assay test. The samples were examined by a double blind approach. The Thamnotoxkit test reacts to pure microcystins, neurotoxins, and crude extracts of cyanobacteria. It can be concluded that the Thamnotox test is an alternative simple, cost-effective method that may replace the mouse bioassay used previously for determination of cyanobacterial toxicity. © 2000 John Wiley & Sons, Inc. Environ Toxicol 15: 549–553, 2000
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