Abstract
Organelle-specific delivery systems are of significant clinical interest. We demonstrate the use of common cyanine dyes Cy3 and Cy5 as vectors for targeting and delivering cargoes to mitochondria in cancer cells. Specifically, conjugation to the dyes can increase cytotoxicity by up to 1000-fold.
Highlights
Organelle-specific delivery systems are of significant clinical interest
We demonstrate the use of common cyanine dyes Cy3 and Cy5 as vectors for targeting and delivering cargoes to mitochondria in cancer cells
While MitoTracker Green contains functional groups for covalent crosslinking to mitochondria, such functionalities do not exist in Cy3
Summary
We demonstrate the use of common cyanine dyes Cy3 and Cy5 as vectors for targeting and delivering cargoes to mitochondria in cancer cells. Upon conjugation of R8 to cyanine dyes, the behaviour of the resulting Cy3–Cy5-R8 (Fig. 1b) was examined by confocal microscopy experiments using HeLa cells treated with different concentrations (1, 2.5 or 10 mM) of Cy3–Cy5-R8 at either 4 or 37 1C.
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