Abstract
Chromobacterium violaceum cells incubated in a chemically defined medium containing methionine and 14C-labeled glycine produced 14C-labeled cyanide. The cyanide carbon produced by the cells was derived from the methyl carbon of glycine. The carboxyl carbon of glycine, and the methyl carbon of methionine, are apparently only indirectly involved in the biogenesis of cyanide. The greatest portion of the carbons of glycine fixed in the cells appeared in the “protein fraction.” The fraction of the glycine carboxyl carbon present in the evolved carbon dioxide remained almost constant for 2–6 hours of incubation and was almost equivalent to the cyanide arising from the methyl carbon of glycine.
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