Cyamopsis tetragonoloba L Taub inoculated with arbuscular mycorrhiza and Pseudomonas fluorescens and treated with mustard oil cake overcome Macrophomina root-rot losses

Abstract

The effects of inoculation of three arbuscular mycorrhizal (AM) fungi namely, Glomus mosseae, Glomus sinuosum, and Scutellospora erythropa in addition to Pseudomonas fluorescens and treatment with mustard oil cake on root-rot disease of Cyamopsis tetragonoloba L plants caused by Macrophomina phaseolina were evaluated under polyhouse conditions for 2 years. Inoculations of an arbuscular mycorrhizal fungi (AMF) in combination with P. fluorescens and mustard oil cake showed best supporting biocontrol system against the root-rot disease besides increasing the plant height, weight, and yield. The biocontrolling efficiency of dual inoculation (AMF + P. fluorescens) was the second best combination followed by AM plus mustard oil cake. Among the three AM fungi, G. mosseae inoculations showed the best results. Different combined AMF inoculations also altered the concentrations of total soluble sugars, orthodihydric phenols, flavonols, and epicuticular wax contents in host plants.