Abstract
It is now becoming obvious that the rapidly developing fields of genomics, proteomics and bioinformatics will prove to be complementary in the comprehensive understanding of biological processes and the rapid development of novel pharmaceuticals. In the field of proteomics [1], the separation sciences will obviously pay a pivotal role in such studies, coupled with highly sensitive and specific down stream analytical techniques such as mass spectrometry. To date 2-D gel electrophoresis has been routinely used as the preferred method for the separation of complex mixtures of cellular proteins. However, it is now realised that this technique has its limitations, in particular for the identification of low abundance proteins (e.g. growth factors, receptors and proteins involved in signal transduction).
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