Abstract
The composition of cutin from stem tissue of Pisum sativum var. Meteor in normal plants and those in which stem extension had been induced with gibberellic acid was determined by capillary GC-chemical ionization mass spectrometry, the first application of the technique to this lipid phytopolymer. Spectra for the TMS ether methyl ester derivatives of the principle monomers 16-hydroxyhecadecanoic acid, hydroxyhexadecanedioic acid and dihydroxyhexadecanoic acid are described, together with those of two ether derivatives of dihydroxypalmitic acid detected as minor components. Relative composition of the monomers and positional isomer composition was essentially identical in cutin from both tissues. Biosynthetic studies with [1 −14C]palmitic acid established an increased rate of cutin biosynthesis during stem extension in response to exogenous gibberellic acid. The composition of the labelled monomers in cutin deposited during stimulated extension was very similar to that in normally extending tissue, and the time course of the response was consistent with induction of the enzymes of cutin monomer biosynthesis.
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