Abstract

The cuticle surfaces of taxonomically widely divergent species of nematodes have been shown to be covered by a surface coat (glycocalyx) that contains carbohydrate. This surface coat is not considered to be part of the nematode cuticle but to be secreted by the nematode onto its surface. In these studies it is shown that the surface coat of the second stage infective dauer larva (DL 2) of the ryegrass nematode, Anguina agrostis (syn. A. funesta), leaves an imprint which is an extremely delicate structure that under normal conditions is not visible under the light microscope. However when treated with the lectin wheat germ agglutinin (WGA) labelled with fluorescein isothiocyanate (FITC) this imprint becomes clearly visible under blue or ultra violet (u.v.) light and resembles a micro finger print.

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