Abstract
The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Aomblyomma cajennense (Fabricius, 1787) tick extract in rabbits. Rabbits were divided into 3 groups of 2 animals each: naive, pre-infested and control. Cutaneous hypersensitivity was induced by intradermal inoculation of 25 microg extract in 0.03 ml of phosphate buffered saline (PBS) in rabbit ears. Control rabbits were inoculated with PBS only. The ear thickness was measured with a Mitutoyo device before and 10 min, 1, 2, 4, 18, 24, 48, 72 and 96 h post-inoculation (PI). Pre-infested rabbits showed an immediate type reaction within the 1st 10 min PI (60% increase in ear thickness) and a delayed reaction (18 h) (85% increase), whereas the naive rabbits showed only the immediate reaction within the 1st 4 h (60% increase). PBS induced only mild reactions. These results point out the crucial role of the cellular immune response of rabbits in the expression of resistance to A. cajennense.
Highlights
Blood-feeding ectoparasites are a major worldwide problem for humans and animals, both as debilitating agents and as vectors of diseases[22]
The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits
In tick–host relationships, a cutaneous hypersensitivity test has been used for different purposes
Summary
Blood-feeding ectoparasites are a major worldwide problem for humans and animals, both as debilitating agents and as vectors of diseases[22]. The nature of the immunological response following tick infestation varies with the tick–host model. This aspect has been comprehensively reviewed by Wikel[19] and Brown[6]. Be shown that this reaction correlates well with the resistance level to ticks, the test could replace the labour-intensive method of counting ticks on individual animals to assess their immune status. It would be helpful in the identification of tick-resistant animals in order to save acaricides and employ selective breeding. The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits
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