Abstract
In the present environment of staggering technical innovations and increasing expectations of quality healthcare it is evident that we need to fine tune our diagnostic abilities in order to fulfil patients� demands for more efficient therapies and augmented quality of life. We are looking for current trends in clinical gynecology that make use of Liquid chromatography tandem mass spectroscopy, technology not yet employed in Romanian laboratories for the clinical practice but that is rapidly becoming the worldwide method of choice for accurate characterization of the hormonal milieu essential for the requirements of women healthcare.
Highlights
In the present environment of staggering technical innovations and increasing expectations of quality healthcare it is evident that we need to fine tune our diagnostic abilities in order to fulfil patients‘ demands for more efficient therapies and augmented quality of life
The various steroid hormones including glucocorticoids, mineralocorticoids, progestins, androgens and estrogens are biosynthesized from cholesterol by the action of enzymes like cytochrome P450 enzymes (CYPs), hydroxysteroid dehydrogenases (HSDs) and steroid reductases
Steroids may be targeted in diverse biological materials and due to the metabolic versatility of the steroids, there are complex combinations of steroidal metabolism products to be analyzed and measurement frequently needs to be preceded by chromatographic procedures
Summary
In the present environment of staggering technical innovations and increasing expectations of quality healthcare it is evident that we need to fine tune our diagnostic abilities in order to fulfil patients‘ demands for more efficient therapies and augmented quality of life. From around 1960, being faster and higher yielding methods, automated immunoassays have been promoted for use in routine clinical laboratories as they skip the separation and derivatization steps.
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