Abstract
With the completion of genome sequencing on more and more organisms,post-genomic era has begun. Gene trapping,as a powerful tool in the functional-genomic era,has been widespreadly applicated in many fields of biomedicine research because of its great merits in cloning,finding novel genes and unraveling their biological functions. The principles,classifications,manipulation procedures,advantages and disadvantages of gene trap technique and its application in developmental biology,novel gene identification, stem cells differentiation,cancer and reproductive medicine research are discussed,which will provide helpful references for relevant researchers.
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