Abstract
Rapid and mass propagation of clean and quality seedlings in horticulture industry becomes a priority for many countries to supply increasing demand of planting materials. Attempt of exploiting the benefit of heterosis from F1 hybrid and overcoming the shortage of planting materials associated with it needs proper intervention. Tissue culture techniques offer disease free, uniform, and large amount of planting materials. The technique is playing a significant role in advancing agriculture in many developing countries in which the current production has to be doubled by 2050. Specially, countries like Ethiopia in which population growth is very high and food insecurity persist, the need for improved production and productivity through the use of modern technologies become apparent. To feed the tremendously growing population, utilization of biotechnology tools such as micro-propagation in agriculture is helpful. Currently, in Ethiopia, somehow there is a fertile ground to exploit the possible advantage which may be acquired from biotechnology as in the case of sugarcane, banana, pineapple etc. micropropagation that boosted the production. This review is therefore to bold out the development of mass-propagation of selected crops and present the current status of protocol optimization of high value horticultural and spice crops. In the meantime, the development of private tissue culture laboratories makes an encouraging advancement where it has to be largely consolidated and highly linked with innovating laboratories to take over the responsibility of multiplying improved varieties. Thus, it can be concluded that despite the development of biotechnology remains at its early stage, promising results were obtained from micro propagation work and if adequate attention given to rapid development of protocol for mass propagation of high value cash crops, there exist untapped potential of biotechnology in Ethiopia.
Highlights
Micro propagation is an in vitro vegetative propagation of plants by tissue culture with the objective to produce large numbers of progeny plants, genetically identical to the parent
In Ethiopia somehow there is a fertile ground to exploit the possible advantage which may be acquired from biotechnology especially from tissue culture
1) To indicate the effort made by research centers and show summary of released micro-propagation protocol for some high value crops in Ethiopia
Summary
Micro propagation is an in vitro vegetative propagation of plants by tissue culture with the objective to produce large numbers of progeny plants, genetically identical to the parent. American Journal of Life Sciences 2021; 9(4): 81-91 was rolled out by the Ethiopian Institute of Agricultural Research (EIAR) in 2000, with the emphasis to optimize protocol for invitro propagation and virus elimination of economically important crops and/or plant species including: banana, cardamom, grapevine, citrus, garlic, potato, geranium, enset, coffee, pineapple, black pepper, sweet potato, cassava, aframomum corrorima and other high value crops [3]. The objective of the current review is to highlight the major achievements of protocol development for mass-propagation of some important high value cash crops and to learn from other similar efforts made elsewhere on plant tissue culture research as well as to compile and briefly present the current state and development of protocol optimization/development in Ethiopia
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