Abstract

UDK: 577.212:574]:007.5(497.6)
 DNA barcoding is a method designed to provide rapid and precise species identifications by using one or more of short gene sequences called barcodes. In most plant and fungi studies, the standard barcodes of choice are three plastid (rbcL, matK and trnH-psbA) and one nuclear (ITS) gene regions. The relatively high, but comparatively conserved rate of sequence evolution of mtDNA has made COI the marker of choice in animals. BOLD is a freely available cloud-based data storage and analysis platform developed with the aim to advance biodiversity science through DNA barcoding species identification. To date, over 6 million barcodes have been deposited in BOLD with 196,000 animal species, 68,000 plant species and 22,000 species of fungi and other organism entries. In this database, there are currently 447 entries for organisms from Bosnia and Herzegovina, which makes 0.0067% of the total number of BOLD entries. According to BOLD statistics, only 1.11% of all organism entries from B&H were submitted by B&H institutions.
 Despite the fact that Bosnia and Herzegovina has valuable natural resources with a high percentage of endemic and autochthonous species, BOLD statistics elucidated the lack of coordinated and systematic DNA barcoding research so far. It is necessary to establish continuous progress of molecular-genetic characterization of these resources in the future. It is up to B&H institutions to decide if they want to continue the practice of submitting the data sporadically or if they will animate the research community to actively participate in this global project.

Highlights

  • DNA barcoding is a method designed to provide rapid and precise species identifications by using one or more of short gene sequences called barcodes

  • DNA barcoding is useful in cases when species identification by a classic morphological method is hindered, e.g. samples in different developmental stages (Kalamujić Stroil et al 2018a), damaged samples, specimens from the gut content (Harms-Tuohy et al, 2016), the fecal sample, and others

  • Identification of species using DNA barcoding methodology begins with the sampling of individuals from different sources

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Summary

Introduction

DNA barcoding is a method designed to provide rapid and precise species identifications by using one or more of short gene sequences called barcodes. One of the most often used global DNA barcode databases is the Barcode of Life Data Systems (BOLD) database which represents a searchable online repository of barcode records, with accompanying data (iBOL, 2017). Barcode of Life Database Systems (BOLD) is a webbased and free-of-charge IT system, which has a flexible data model that makes it suitable for projects involving more research teams (Ratnasingham & Hebert, 2007; Kalamujić Stroil et al 2018b).

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