Abstract
BACKGROUND: The Monarda fistulosa L. is successfully cultivated in Russia. The herbs of Thymus vulgaris, Thymus serpyllum and Origanum vulgare are used as agents with antimicrobial, antifungal and anti-inflammatory effects, it is due to the presence of thymol and carvacrol in the raw material. However Monarda fistulosa is not inferior to the listed plants.
 AIM: The aim is to study the chemical composition, to identify significant morphological, anatomical and chemical signs of raw materials for diagnostics, as well as to develop methods to standardize the herb of Monarda fistulosa in accordance with the modern requirements of pharmaceutical analysis.
 MATERIALS AND METHODS: The herb of Monarda fistulosa was harvested during the period of mass flowering in the Botanical Garden of Samara University in 2016-2019. Morphological and anatomical examination was carried out by the method of light microscopy in transmitted and reflected light. Luminescence microscopy was carried out on a luminescence microscope Altami LUM 2. Individual substances were isolated by column chromatography. The 13C NMR spectra was evaluated by means of the instrument Bruker DRX 500, and Bruker AM 300 was used to record 1H NMR spectra. Mass spectra were recorded on mass spectrometer Kratos MS-30; UV spectra were recorded using a Specord 40 spectrophotometer (Analytik Jena).
 RESULTS: This paper discusses the results of research in the field of standardization of raw materials for Monarda fistulosa. As a result of the experimental work, characteristic anatomical and morphological features of the plant were determined. For the first time, the dominant flavonoids like isoroifolin, linarin, and other flavonoids like didimine, acacetin, apigenin were isolated from the herb of the studied raw material. The authors isolated a new natural compound 5-O-rutinoside apigenin from the herb of Monarda fistulosa. Methods for the qualitative analysis of the herb Monarda fistulosa have been developed using thin-layer chromatography (in the presence of thymol and rutin) and spectrophotometry (absorption maxima at a wavelength of 270 2 and 330 2 nm). A method has been developed for the quantitative determination of the total flavonoid content in the herb Monarda fistulosa L. using differential spectrophotometry at the analytical wavelength of 394 nm.
 CONCLUSIONS: The obtained results were used in the drafting of the pharmacopoeial monograph for the new type of raw material Monarda fistulosa L. herb.
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